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FineTest

SKU:FNab09105

anti- Tubulin-beta antibody

anti- Tubulin-beta antibody

Regular price $330.00 USD
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size

anti- Tubulin-beta antibody

Product Name

Tubulin-beta antibody

Size

100µg

Form

liquid

Purification

Protein A+G purification

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha and beta tubulins have been widely used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

IMMUNOGEN INFORMATION

Immunogen

tubulin, beta 3

Synonyms

Beta tubulin, MC1R, TUBB, Tubulin-beta

Observed MW

50 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:10000-1:50000; IF: 1:20-1:200; IP: 1:500-1:2000; IHC: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

Q13509

IMAGES

 

Immunohistochemistry of paraffin-embedded human brain tissue slide using FNab09105(Tubulin-beta Antibody) at dilution of 1:400
Immunofluorescent analysis of HepG2 cells using FNab09105 ( Tubulin-beta Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)
IP Result of anti-Tubulin-beta (IP: FNab09105, 5ug; Detection: FNab09105 1:1000) with HeLa cells lysate 880ug.
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with FNab09105(Tubulin-beta Antibody) at dilution of 1:10000

 

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