anti- UBE2O antibody

anti- UBE2O antibody

Product Name

UBE2O antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins(PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity(PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis(PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal(NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location(PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASH1, leading to promote endosomal F-actin assembly(PubMed:23452853).

IMMUNOGEN INFORMATION

Immunogen

ubiquitin-conjugating enzyme E2O

Synonyms

E2-230K, FLJ12878, KIAA1734, UBE2O, Ubiquitin carrier protein O, Ubiquitin protein ligase O

Observed MW

141 kDa

APPLICATION

Tested Application

ELISA, WB

Recommended Dilution

WB: 1:200-1:2000