anti- CASP3 antibody

Our Anti-CASP3 Antibody is a versatile research tool for scientists exploring apoptosis, a crucial process in cell biology and disease. Caspase-3 (CASP3) is a central player in the apoptotic cascade, responsible for cleaving vital cellular substrates during programmed cell death.

This antibody is meticulously designed to selectively recognize CASP3, ensuring the specificity of your experiments. It is compatible with various applications, including Western blotting and immunofluorescence, offering flexibility in your research.

With the 100µg vial size, you'll have an ample supply of this high-quality antibody for multiple experiments, ensuring cost-effectiveness and consistent results. Gain deeper insights into the intricate mechanisms of apoptosis and CASP3's role in regulating cell fate with confidence, knowing you have a trusted research partner.

Product Name

CASP3 antibody

Size

100µg

Form

liquid

Purification

protein A+G purified

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

IMMUNOGEN INFORMATION

Immunogen

caspase 3, apoptosis-related cysteine peptidase

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

Observed MW

32-35 kDa, 19 kDa, 17 kDa

APPLICATION

Tested Application

ELISA, IHC, WB,IF

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:50-1:300; IF: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

P42574

IMAGES

Immunohistochemistry of paraffin-embedded human breast cancer using FNab09938(CASP3 antibody) at dilution of 1:200

Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with FNab09938 (CASP3 antibody) at dilution of 1:3000