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FNab09940

anti- HIF1a antibody

anti- HIF1a antibody

$330.00 USD
$330.00 USD
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size

anti- HIF1a antibody

Product Name

HIF1a antibody

Size

100µg

Form

liquid

Purification

protein A+G purified

Purity

≥95% as determined by SDS-PAGE

Host

Mouse

Clonality

monoclonal

Isotype

IgG1

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia. The calculated molecular weight of HIF1a is 93 kDa, but the modified protein HIF1a is about 110-120kDa (PMID: 11698256, .PMID: 7539918).

IMMUNOGEN INFORMATION

Immunogen

hypoxia inducible factor 1, alpha subunit(basic helix-loop-helix transcription factor)

Synonyms

BHLHE78, MOP1, PASD8

Observed MW

120 kDa

APPLICATION

Tested Application

ELISA, IF, WB

Recommended Dilution

WB: 1:1000-1:10000; IF: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

Q16665

IMAGES

 

Immunofluorescent analysis of ( -20°C Ethanol) fixed Cobalt Chloride treated HeLa cells using FNab09940 (HIF1a antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L)
Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab09940(HIF1a Antibody) at dilution of 1:5000

 

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