anti- c-Kit/CD117 antibody

Uncover the diverse functions of c-Kit/CD117 with our Anti-c-Kit/CD117 Antibody, available in a 100µg size as a polyclonal antibody. This versatile antibody is your ideal tool for investigating c-Kit/CD117, a receptor tyrosine kinase that plays pivotal roles in hematopoiesis, mast cell development, and gastrointestinal motility.

Engineered for precision and reliability, this polyclonal antibody ensures exceptional sensitivity in applications such as flow cytometry, immunohistochemistry, and immunoprecipitation. Whether you're probing c-Kit/CD117's roles in stem cell biology, allergy responses, or its interactions with regulatory molecules, this antibody enhances your research capabilities.

c-Kit/CD117 is an essential receptor in various cellular processes, impacting hematopoietic stem cell survival, mast cell differentiation, and gastrointestinal motility. Mutations in c-Kit are associated with diseases such as systemic mastocytosis and gastrointestinal stromal tumors (GISTs).

With our Anti-c-Kit/CD117 Antibody, you can delve into c-Kit/CD117's multifaceted functions, explore its contributions to disease pathogenesis, and potentially advance research in hematological disorders and targeted therapies. Elevate your research in understanding the roles of c-Kit/CD117 in cellular processes with this precision polyclonal antibody.

Product Name

c-Kit/CD117 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK(isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

IMMUNOGEN INFORMATION

Immunogen

Mast/stem cell growth factor receptor Kit

Synonyms

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit

Observed MW

120 kDa, 145 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:200-1:2000; IHC: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

P10721

Immunohistochemistry of paraffin-embedded rat ovary tissue slide using FNab09971(C-KIT Antibody) at dilution of 1:100

MCF7 cells were subjected to SDS PAGE followed by western blot with FNab09971(c-Kit/CD117 antibody) at dilution of 1:200