anti- Caspase 3 antibody

Our Anti-Caspase 3 Antibody (Polyclonal, 100µg) is a versatile tool for researchers investigating apoptosis, a fundamental process in cellular biology. Caspase 3, also known as CPP32, plays a pivotal role in orchestrating cell death. This polyclonal antibody is designed to specifically target Caspase 3, ensuring precision in your experiments.

With 100µg of antibody per vial, you'll have an ample supply for multiple experiments, reducing the need for frequent reordering. This antibody is produced in a rabbit host and offers excellent sensitivity and specificity, making it ideal for various applications, including Western blotting, immunohistochemistry, and flow cytometry.

Our Anti-Caspase 3 Antibody (Polyclonal, 100µg) empowers researchers to explore apoptosis pathways with confidence. Its high-quality performance guarantees the accuracy of your results, contributing to the advancement of scientific knowledge in the fields of cell biology and disease research.

Product Name

Caspase 3 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. The antibody can recognize p17 and p32 of Caspase 3.

IMMUNOGEN INFORMATION

Immunogen

caspase 3, apoptosis-related cysteine peptidase

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

Observed MW

32-35 kDa, 17 kDa

APPLICATION

Tested Application

ELISA, WB, IHC, IF

Recommended Dilution

WB: 1:200-1:2000; IHC: 1:50-1:500; IF: 1:50-1:200

UNIPROT INFORMATION

UniProt ID

P42574

IMAGES

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using FNab10013(CASP3 Antibody) at dilution of 1:100

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using FNab10013 (caspase 3 antibody) at dilution of 1:50 and FITC-Conjugated Goat Anti-rabbit IgG(H+L)

Jurkat cells were subjected to SDS PAGE followed by western blot with FNab10013(CASP3 antibody) at dilution of 1:1000