anti- DNA2 antibody

The anti-DNA2 antibody, available in a 100µg x2 size, is a powerful research tool designed for scientists investigating DNA2, a multifunctional nuclease/helicase known for its crucial roles in DNA replication, repair, and maintenance. This polyclonal antibody offers high specificity and sensitivity, making it suitable for various applications, including immunohistochemistry and Western blotting.

DNA2 is a key player in DNA metabolism and genome stability. Researchers can utilize this antibody to explore the expression patterns and functions of DNA2, contributing to our understanding of its roles in maintaining genomic integrity. The availability of 100µg x2 per vial ensures an ample supply for multiple experiments, making it a cost-effective and flexible choice for researchers.

This polyclonal antibody undergoes rigorous quality control to ensure reliable and consistent results, providing researchers with the confidence they need to advance their scientific investigations effectively.

In summary, the anti-DNA2 antibody is a high-quality polyclonal reagent designed to empower researchers in their studies of DNA replication and repair mechanisms. By investigating DNA2, scientists can make valuable contributions to our understanding of essential cellular processes and their implications in health and disease.

Product Name

DNA2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex(RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break(DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA(ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

IMMUNOGEN INFORMATION

Immunogen

DNA replication helicase 2 homolog

Synonyms

DNA2, DNA2L, KIAA0083

Observed MW

127 kDa

APPLICATION

Tested Application

ELISA, WB, IHC

Recommended Dilution

WB: 1:500-1:2000; IHC: 1:50-1:500

UNIPROT INFORMATION

UniProt ID

P51530

IMAGES

Immunohistochemistry of paraffin-embedded human colon cancer using FNab10066(DNA2 antibody) at dilution of 1:100

Rat thymus were subjected to SDS PAGE followed by western blot with FNab10066(DNA2 antibody) at dilution of 1:1000.