Recombinant Human CXCL1

CINC1; CINC-1; CXCL1; GRO alpha; KC; MGSA-alpha

Product Name

Recombinant Human CXCL1

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

35-107

Mol. Weight

9.7 KDa

Tags

N-terminal His Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. The ED50 for this effect is 0.5-2 µg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

CINC1, CINC-1, CXCL1, GRO alpha, KC, MGSA-alpha