Recombinant Human UBE2E3

Product Name

Recombinant Human UBE2E3

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

1-207

Mol. Weight

22.7 kDa

Tags

N-terminal His Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

Homologous to yeast UBC4/5, UB2E3_HUMAN, UBC E4, Ubc M2, UBC4/5 homolog, UBCE 4, UBCE4, UBCH 9, UbcH9, UbcM2, UBE2 E3, UBE2E3, Ubiquitin carrier protein, Ubiquitin carrier protein E3, Ubiquitin conjugating enzyme E2 23 kDa, Ubiquitin conjugating enzyme E2 E3