Recombinant Human NCE2

Product Name

Recombinant Human NCE2

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

1-185

Mol. Weight

TBD

Tags

determined during production processN-terminal His Tag or N-terminal His-IF2DI Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.

Alternative Names

NCE2, NEDD8 carrier protein UBE2F, NEDD8 conjugating enzyme, NEDD8 conjugating enzyme UBE2F, NEDD8 protein ligase UBE2F, NEDD8-conjugating enzyme 2, NEDD8-conjugating enzyme UBE2F, ube2f, UBE2F_HUMAN, Ubiquitin conjugating enzyme E2F, Ubiquitin conjugating enzyme E2F (putative), Ubiquitin-conjugating enzyme E2 F