Recombinant Human SIAH1

Product Name

Recombinant Human SIAH1

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

90-282

Mol. Weight

21.1 kDa

Tags

determined during production processN-terminal His Tag or N-terminal His-IF2DI Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

E3 ubiquitin-protein ligase SIAH1, FLJ08065, hSIAH1, HUMSIAH, Seven in absentia homolog 1, Seven in absentia homolog 1 (Drosophila), Siah 1, Siah 1a, Siah E3 ubiquitin protein ligase 1, Siah-1, Siah-1a, Siah1, SIAH1_HUMAN, SIAH1A, Ubiquitin ligase SIAH1