Recombinant Human SFT

Product Name

Recombinant Human SFT

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

1-147

Mol. Weight

16.1 kDa

Tags

determined during production processN-terminal His Tag or N-terminal His-IF2DI Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

E2(17)KB1, SFT, Stimulator of Fe transport, UB2D1_HUMAN, UBC 4/5, UBC4/5, UBC4/5 homolog, UBC4/5, S. cerevisiae, homolog of, UBCH 5, UBCH 5A, UbcH5, UBCH5A, Ube2d1, Ubiquitin carrier protein, Ubiquitin carrier protein D1, Ubiquitin conjugating enzyme E2 17 kDa 1