Recombinant Mouse CXCL5

Product Name

Recombinant Mouse CXCL5

Size

50μg, 200μg, 1mg

Species

Mouse

Host

E.Coli

Expression Region

41-132

Mol. Weight

10 kDa

Tags

N-terminal His Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR2. The ED50 for this effect is 5-20 ng/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

AMCFII, C-X-C motif chemokine 5, C-X-C motif chemokine ligand 5, Chemokine (C X C motif) ligand 5, chemokine (C-X-C motif) ligand 5, Cxcl5, CXCL5_HUMAN, ENA 78, ENA-78 (8-78), ENA-78(1-78), ENA-78(9-78), ENA78, Epithelial derived neutrophil activating protein 78