Recombinant Mouse Macrophage Inflammatory1 alpha

Product Name

Recombinant Mouse Macrophage Inflammatory1 alpha

Size

50μg, 200μg, 1mg

Species

Mouse

Host

E.Coli

Expression Region

24-92

Mol. Weight

7.5 kDa

Tags

determined during production processN-terminal His Tag or N-terminal His-IF2DI Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 10-30 ng/mL

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

C C motif chemokine 3, CCL 3, CCL3, CCL3_HUMAN, Chemokine (C C motif) ligand 3, Chemokine C C motif ligand 3, Chemokine ligand 3, G0/G1 switch regulatory protein 19 1, G0/G1 switch regulatory protein 19-1, G0S19 1, G0S19 1 protein, Heparin binding chemotaxis protein, L2G25B, LD78 alpha