Recombinant Human IGFBP1

Product Name

Recombinant Human IGFBP1

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

26-259

Mol. Weight

25.6 kDa

Tags

N-terminal His Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

AFBP, Alpha pregnancy associated endometrial globulin, Amniotic fluid binding protein, Binding protein 25, Binding protein 26, Binding protein 28, Growth hormone independent binding protein, hIGFBP 1, hIGFBP1, IBP 1, IBP-1, IBP1, IBP1_HUMAN, IGF binding protein 1, IGF BP25