Recombinant Human IL-5

Product Name

Recombinant Human IL-5

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

20-134

Mol. Weight

15 kDa

Tags

N-terminal His Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.4-1 ng/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

B-cell differentiation factor I, Colony stimulating factor, EDF, Eosinophil differentiation factor, IL-5, IL5, IL5_HUMAN, Interleukin 5, Interleukin 5 (colony stimulating factor, eosinophil), Interleukin-5, T Cell Replacing Factor, T-cell replacing factor, TRF