Recombinant Human Wnt1

Product Name

Recombinant Human Wnt1

Size

50μg, 200μg, 1mg

Species

Human

Host

E.Coli

Expression Region

28-370

Mol. Weight

58.9 kDa

Tags

N-terminal His-IF2DI Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

BMND16, INT1, OI15, oncogene Int1, Proto oncogene protein Wnt 1, Proto-oncogene Int-1 homolog, Proto-oncogene Wnt-1, Wingless type MMTV integration site family member 1, wingless-type MMTV integration site family, member 1 (oncogene INT1), Wnt 1, wnt1, WNT1_HUMAN