Recombinant Mouse IL-1 alpha

Product Name

Recombinant Mouse IL-1 alpha

Size

50μg, 200μg, 1mg

Species

Mouse

Host

E.Coli

Expression Region

115-270

Mol. Weight

17.1 kDa

Tags

N-terminal His Tag

Purity

Greater than 95% as determined by SDS-PAGE

Application

Western Blot, ELISA

Activity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells.The ED50 for this effect is 10-50 pg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4

Reconstitution

Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex

Storage

The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze/thaw cycle.

Alternative Names

BAF, FAF, Hematopoietin 1, Hematopoietin-1, IL 1 alpha, IL 1A, IL-1 alpha, Il-1a, IL1, IL1 ALPHA, IL1A, IL1A_HUMAN, IL1F1, Interleukin 1 alpha, Interleukin-1 alpha, Interleukin1 alpha, LAF, LEM, Preinterleukin 1 alpha, Pro interleukin 1 alpha