FineTest
SKU:EH2874
Human Collagenase I(Collagenase I ) ELISA Kit
Human Collagenase I(Collagenase I ) ELISA Kit
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Product Name
Human Collagenase I(Collagenase I ) ELISA Kit
Alias
Collagenase I ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Collagenase I ELISA Kit allows for the in vitro quantitative determination of Collagenase I concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Collagenase I and the recovery rates were calculated by comparing the measured value to the expected amount of Collagenase I in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
95 |
|
EDTA plasma(n=5) |
86-103 |
93 |
|
heparin plasma(n=5) |
93-102 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Collagenase I and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-104% |
85-105% |
86-103% |
|
EDTA plasma(n=5) |
82-91% |
87-96% |
82-97% |
|
heparin plasma(n=5) |
80-100% |
85-99% |
82-94% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
