FineTest
SKU:EH12548
Human SPHK1(Sphingosine Kinase 1) ELISA Kit
Human SPHK1(Sphingosine Kinase 1) ELISA Kit
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Product Name
Human SPHK1(Sphingosine Kinase 1) ELISA Kit
Alias
Sphingosine kinase 1 ELISA Kit, SK 1 ELISA Kit, SPK 1 ELISA Kit, Acetyltransferase SPHK1 ELISA Kit, SK1 ELISA Kit, SPHK ELISA Kit, SPK ELISA Kit, SPHK1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SPHK1 ELISA Kit allows for the in vitro quantitative determination of SPHK1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SPHK1 and the recovery rates were calculated by comparing the measured value to the expected amount of SPHK1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
97 |
|
EDTA plasma(n=5) |
86-102 |
94 |
|
heparin plasma(n=5) |
87-103 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPHK1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
92-98% |
91-100% |
89-100% |
|
EDTA plasma(n=5) |
87-100% |
86-93% |
86-94% |
|
heparin plasma(n=5) |
85-92% |
87-97% |
85-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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