FineTest
SKU:EH3912
Human TRH (Thyrotropin Releasing Hormone) ELISA Kit
Human TRH (Thyrotropin Releasing Hormone) ELISA Kit
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Product Name
Human TRH (Thyrotropin Releasing Hormone) ELISA Kit
Alias
TRH ELISA Kit, Thyrotropin Releasing Hormone ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TRH ELISA Kit allows for the in vitro quantitative determination of TRH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.78-50pg/ml
Sensitivity
< 0.46pg/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TRH and the recovery rates were calculated by comparing the measured value to the expected amount of TRH in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-99 |
91 |
|
EDTA plasma(n=5) |
92-99 |
96 |
|
heparin plasma(n=5) |
87-103 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-103% |
86-103% |
86-105% |
|
EDTA plasma(n=5) |
87-99% |
82-98% |
84-99% |
|
heparin plasma(n=5) |
86-100% |
84-95% |
85-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
