FineTest
SKU:ESH0092
Sheep S100B(S100 Calcium Binding Protein B) ELISA Kit
Sheep S100B(S100 Calcium Binding Protein B) ELISA Kit
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Product Name
Sheep S100B(S100 Calcium Binding Protein B) ELISA Kit
Alias
S100B ELISA Kit, S100 Calcium Binding Protein B ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
S100B ELISA Kit allows for the in vitro quantitative determination of S100B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Sheep
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of S100B and the recovery rates were calculated by comparing the measured value to the expected amount of S100B in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-99 |
93 |
|
EDTA plasma(n=5) |
85-100 |
95 |
|
heparin plasma(n=5) |
93-103 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
92-101% |
89-102% |
86-104% |
|
EDTA plasma(n=5) |
82-88% |
83-97% |
86-101% |
|
heparin plasma(n=5) |
85-97% |
81-93% |
83-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
