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Rat LGR-5(leucine-rich repeat-containing g-protein coupled receptor 5) ELISA Kit
Rat LGR-5(leucine-rich repeat-containing g-protein coupled receptor 5) ELISA Kit
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Product Name
Rat LGR-5(leucine-rich repeat-containing g-protein coupled receptor 5) ELISA Kit
Alias
Leucine-rich repeat-containing G-protein coupled receptor 5 ELISA Kit, Lgr5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
LGR-5 ELISA Kit allows for the in vitro quantitative determination of Lgr5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Lgr5 and the recovery rates were calculated by comparing the measured value to the expected amount of Lgr5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-103 |
97 |
|
EDTA plasma(n=5) |
88-104 |
96 |
|
heparin plasma(n=5) |
89-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lgr5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
92-105% |
85-96% |
86-97% |
|
EDTA plasma(n=5) |
83-93% |
82-98% |
83-88% |
|
heparin plasma(n=5) |
87-100% |
82-97% |
83-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
