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SKU:EH4453

Human 10-HETE(10-Hydroxyeicosatetraenoic Acid) ELISA Kit

Human 10-HETE(10-Hydroxyeicosatetraenoic Acid) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Human 10-HETE(10-Hydroxyeicosatetraenoic Acid) ELISA Kit

Alias

10-HETE ELISA Kit10-Hydroxyeicosatetraenoic Acid ELISA Kit

Detection Method

Competitive ELISA, Coated with Antigen

Application

 10-HETE ELISA Kit allows for the in vitro quantitative determination of 10-HETE concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of 10-HETE and the recovery rates were calculated by comparing the measured value to the expected amount of 10-HETE in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

97-102

100

EDTA plasma(n=5)

90-105

97

heparin plasma(n=5)

87-105

93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 10-HETE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

85-104%

86-92%

86-94%

EDTA plasma(n=5)

85-100%

84-99%

86-93%

heparin plasma(n=5)

81-95%

81-95%

82-96%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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