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FineTest

SKU:EH4451

Human SIRT3(NAD-dependent ADP-ribosyltransferase sirtuin-4) ELISA Kit

Human SIRT3(NAD-dependent ADP-ribosyltransferase sirtuin-4) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Human SIRT3(NAD-dependent ADP-ribosyltransferase sirtuin-4) ELISA Kit

Alias

Slit homolog 3 protein ELISA KitSlit-3 ELISA KitMultiple epidermal growth factor-like domains protein 5 ELISA KitMultiple EGF-like domains protein 5 ELISA KitSLIT3 ELISA KitKIAA0814 ELISA KitMEGF5 ELISA KitSLIL2 ELISA KitUNQ691 ELISA KitPRO1336 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

 SIRT3 ELISA Kit allows for the in vitro quantitative determination of SIRT3 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

UniProt ID

O75094

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SIRT3 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT3 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

86-101

94

EDTA plasma(n=5)

85-103

97

heparin plasma(n=5)

93-104

98

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

85-105%

95-100%

87-95%

EDTA plasma(n=5)

82-101%

85-97%

86-100%

heparin plasma(n=5)

84-98%

81-98%

80-94%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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