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SKU:EH4451
Human SIRT3(NAD-dependent ADP-ribosyltransferase sirtuin-4) ELISA Kit
Human SIRT3(NAD-dependent ADP-ribosyltransferase sirtuin-4) ELISA Kit
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Product Name
Human SIRT3(NAD-dependent ADP-ribosyltransferase sirtuin-4) ELISA Kit
Alias
Slit homolog 3 protein ELISA Kit, Slit-3 ELISA Kit, Multiple epidermal growth factor-like domains protein 5 ELISA Kit, Multiple EGF-like domains protein 5 ELISA Kit, SLIT3 ELISA Kit, KIAA0814 ELISA Kit, MEGF5 ELISA Kit, SLIL2 ELISA Kit, UNQ691 ELISA Kit, PRO1336 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SIRT3 ELISA Kit allows for the in vitro quantitative determination of SIRT3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SIRT3 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-101 |
94 |
|
EDTA plasma(n=5) |
85-103 |
97 |
|
heparin plasma(n=5) |
93-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
95-100% |
87-95% |
|
EDTA plasma(n=5) |
82-101% |
85-97% |
86-100% |
|
heparin plasma(n=5) |
84-98% |
81-98% |
80-94% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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