FineTest
SKU:EH13962
Human AKT1S1 (Proline-rich AKT1 substrate 1) ELISA Kit
Human AKT1S1 (Proline-rich AKT1 substrate 1) ELISA Kit
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Product Name
Human AKT1S1 (Proline-rich AKT1 substrate 1) ELISA Kit
Alias
Proline-rich AKT1 substrate 1 ELISA Kit, 40 kDa proline-rich AKT substrate ELISA Kit, AKT1S1 ELISA Kit, PRAS40 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
AKT1S1 ELISA Kit allows for the in vitro quantitative determination of AKT1S1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of AKT1S1 and the recovery rates were calculated by comparing the measured value to the expected amount of AKT1S1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-100 |
95 |
|
EDTA plasma(n=5) |
87-101 |
96 |
|
heparin plasma(n=5) |
91-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKT1S1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-99% |
87-102% |
85-102% |
|
EDTA plasma(n=5) |
87-94% |
85-100% |
85-101% |
|
heparin plasma(n=5) |
81-99% |
84-100% |
80-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
