FineTest
SKU:EH8039
Human DPPA4 (Developmental pluripotency-associated protein 4) ELISA Kit
Human DPPA4 (Developmental pluripotency-associated protein 4) ELISA Kit
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Product Name
Human DPPA4 (Developmental pluripotency-associated protein 4) ELISA Kit
Alias
Developmental pluripotency-associated protein 4 ELISA Kit, DPPA4 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
DPPA4 ELISA Kit allows for the in vitro quantitative determination of DPPA4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of DPPA4 and the recovery rates were calculated by comparing the measured value to the expected amount of DPPA4 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-105 |
100 |
|
EDTA plasma(n=5) |
87-105 |
98 |
|
heparin plasma(n=5) |
91-103 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DPPA4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-99% |
87-105% |
87-104% |
|
EDTA plasma(n=5) |
83-100% |
84-97% |
82-100% |
|
heparin plasma(n=5) |
85-98% |
85-99% |
91-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
