FineTest
SKU:ER1922
Rat ATG12 ( autophagy protein 12 ) ELISA Kit
Rat ATG12 ( autophagy protein 12 ) ELISA Kit
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Product Name
Rat ATG12 ( autophagy protein 12 ) ELISA Kit
Alias
Ubiquitin-like protein ATG12 ELISA Kit, Autophagy-related protein 12 ELISA Kit, APG12-like ELISA Kit, Atg12 ELISA Kit, Apg12l ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ATG12 ELISA Kit allows for the in vitro quantitative determination of ATG12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ATG12 and the recovery rates were calculated by comparing the measured value to the expected amount of ATG12 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-105 |
95 |
|
EDTA plasma(n=5) |
85-98 |
92 |
|
heparin plasma(n=5) |
85-99 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATG12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-105% |
87-103% |
86-104% |
|
EDTA plasma(n=5) |
84-95% |
86-99% |
82-100% |
|
heparin plasma(n=5) |
91-100% |
82-95% |
86-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
