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SKU:EM1917
Mouse Gpbar1 (G-protein coupled bile acid receptor 1) ELISA Kit
Mouse Gpbar1 (G-protein coupled bile acid receptor 1) ELISA Kit
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Product Name
Mouse Gpbar1 (G-protein coupled bile acid receptor 1) ELISA Kit
Alias
G-protein coupled bile acid receptor 1 ELISA Kit, Membrane-type receptor for bile acids ELISA Kit, M-BAR ELISA Kit, Gpbar1 ELISA Kit, Tgr5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Gpbar1 ELISA Kit allows for the in vitro quantitative determination of Gpbar1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Mouse
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Gpbar1 and the recovery rates were calculated by comparing the measured value to the expected amount of Gpbar1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
93 |
|
EDTA plasma(n=5) |
93-101 |
97 |
|
heparin plasma(n=5) |
89-93 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gpbar1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-103% |
86-104% |
88-105% |
|
EDTA plasma(n=5) |
84-101% |
82-100% |
85-100% |
|
heparin plasma(n=5) |
83-99% |
82-99% |
80-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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