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SKU:ER1913
Rat MLCK/MYLK (Myosin light chain kinase) ELISA Kit
Rat MLCK/MYLK (Myosin light chain kinase) ELISA Kit
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Product Name
Rat MLCK/MYLK (Myosin light chain kinase) ELISA Kit
Alias
Myosin light chain kinase 2, skeletal/cardiac muscle ELISA Kit, MLCK2 ELISA Kit, Mylk2 ELISA Kit, Myosin light chain kinase ELISA Kit, MLCK ELISA Kit, MYLK ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MLCK/MYLK ELISA Kit allows for the in vitro quantitative determination of MLCK/MYLK concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Rat
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of MLCK/MYLK and the recovery rates were calculated by comparing the measured value to the expected amount of MLCK/MYLK in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-105 |
94 |
|
EDTA plasma(n=5) |
87-105 |
97 |
|
heparin plasma(n=5) |
86-102 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MLCK/MYLK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-101% |
89-97% |
91-103% |
|
EDTA plasma(n=5) |
86-99% |
86-101% |
83-97% |
|
heparin plasma(n=5) |
86-100% |
81-98% |
83-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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