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EM1464

Mouse 25 Hydroxy Vitamin D (25 Hydroxy Vitamin D) ELISA Kit

Mouse 25 Hydroxy Vitamin D (25 Hydroxy Vitamin D) ELISA Kit

$450.00 USD
$450.00 USD
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size
Sensitivity
Range

Product Name

Mouse 25 Hydroxy Vitamin D (25 Hydroxy Vitamin D) ELISA Kit

Alias

25 Hydroxy Vitamin D ELISA Kit

Detection Method

Competitive ELISA, Coated with Antigen

Application

 25 Hydroxy Vitamin D  ELISA Kit allows for the in vitro quantitative determination of 25 Hydroxy Vitamin D concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

3.13-200ng/ml

Sensitivity

< 1.875ng/ml

Species

Mouse

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of 25 Hydroxy Vitamin D and the recovery rates were calculated by comparing the measured value to the expected amount of 25 Hydroxy Vitamin D in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

89-105

98

EDTA plasma(n=5)

88-105

95

heparin plasma(n=5)

90-100

96

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25 Hydroxy Vitamin D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

88-100%

87-105%

90-105%

EDTA plasma(n=5)

88-98%

82-101%

82-100%

heparin plasma(n=5)

83-99%

81-99%

86-90%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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