FineTest
SKU:EU3131
12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit
12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit
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Product Name
12-HETE (12-Hydroxyeicosatetraenoic Acid) ELISA Kit
Alias
12-HETE ELISA Kit, 12-Hydroxyeicosatetraenoic Acid ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
12-HETE ELISA Kit allows for the in vitro quantitative determination of 12-HETE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Universal
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 12-HETE and the recovery rates were calculated by comparing the measured value to the expected amount of 12-HETE in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
95 |
|
EDTA plasma(n=5) |
87-98 |
94 |
|
heparin plasma(n=5) |
88-101 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 12-HETE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-104% |
85-101% |
94-105% |
|
EDTA plasma(n=5) |
83-93% |
82-100% |
82-101% |
|
heparin plasma(n=5) |
80-97% |
84-99% |
84-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
