FineTest
SKU:EH0364
Human CYFRA21-1 (CytokeRatin Fragment Antigen 21-1) ELISA Kit
Human CYFRA21-1 (CytokeRatin Fragment Antigen 21-1) ELISA Kit
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Product Name
Human CYFRA21-1/Cytokeratin-19(CytokeRatin Fragment Antigen 21-1) ELISA Kit
Alias
CYFRA21-1 ELISA Kit, CytokeRatin Fragment Antigen 21-1 ELISA Kit, Cytokeratin-19 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CYFRA21-1/Cytokeratin-19 ELISA Kit allows for the in vitro quantitative determination of CYFRA21-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CYFRA21-1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYFRA21-1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-95 |
90 |
|
EDTA plasma(n=5) |
84-103 |
92 |
|
heparin plasma(n=5) |
84-99 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYFRA21-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
82-100% |
81-99% |
84-103% |
|
EDTA plasma(n=5) |
91-99% |
91-101% |
90-96% |
|
heparin plasma(n=5) |
91-100% |
92-99% |
93-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
