FineTest
SKU:EH1396
Human CA50 (CarbohydRate Antigen 50) ELISA Kit
Human CA50 (CarbohydRate Antigen 50) ELISA Kit
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Product Name
Human CA50(CarbohydRate Antigen 50) ELISA Kit
Alias
CA50 ELISA Kit, CarbohydRate Antigen 50 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CA50 ELISA Kit allows for the in vitro quantitative determination of CA50 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CA50 and the recovery rates were calculated by comparing the measured value to the expected amount of CA50 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-104 |
97 |
|
EDTA plasma(n=5) |
85-105 |
91 |
|
heparin plasma(n=5) |
93-103 |
100 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CA50 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-101% |
93-104% |
86-103% |
|
EDTA plasma(n=5) |
84-96% |
92-99% |
86-98% |
|
heparin plasma(n=5) |
88-96% |
85-100% |
81-94% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
