FineTest
SKU:EH13209
Human TRIM5 (Tripartite motif-containing protein 5) ELISA Kit
Human TRIM5 (Tripartite motif-containing protein 5) ELISA Kit
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Product Name
Human TRIM5(Tripartite motif-containing protein 5) ELISA Kit
Alias
Tripartite motif-containing protein 5 (EC:2.3.2.27) ELISA Kit, RING finger protein 88 ELISA Kit, RING-type E3 ubiquitin transferase TRIM5 ELISA Kit, TRIM5 ELISA Kit, RNF88 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TRIM5 ELISA Kit allows for the in vitro quantitative determination of TRIM5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TRIM5 and the recovery rates were calculated by comparing the measured value to the expected amount of TRIM5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-95 |
90 |
|
EDTA plasma(n=5) |
91-96 |
94 |
|
heparin plasma(n=5) |
86-102 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRIM5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
99-104% |
92-102% |
86-104% |
|
EDTA plasma(n=5) |
83-98% |
82-101% |
83-96% |
|
heparin plasma(n=5) |
84-99% |
85-95% |
85-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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