FineTest
SKU:EH12013
Human RSPO3 (R-spondin-3) ELISA Kit
Human RSPO3 (R-spondin-3) ELISA Kit
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Product Name
Human RSPO3 (R-spondin-3) ELISA Kit
Alias
R-spondin-3 ELISA Kit, Protein with TSP type-1 repeat ELISA Kit, hPWTSR ELISA Kit, Roof plate-specific spondin-3 ELISA Kit, hRspo3 ELISA Kit, Thrombospondin type-1 domain-containing protein 2 ELISA Kit, RSPO3 ELISA Kit, PWTSR ELISA Kit, THSD2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
RSPO3 ELISA Kit allows for the in vitro quantitative determination of RSPO3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of RSPO3 and the recovery rates were calculated by comparing the measured value to the expected amount of RSPO3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
97-103 |
100 |
|
EDTA plasma(n=5) |
95-105 |
99 |
|
heparin plasma(n=5) |
95-100 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RSPO3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-101% |
91-105% |
90-102% |
|
EDTA plasma(n=5) |
89-101% |
90-96% |
95-101% |
|
heparin plasma(n=5) |
97-99% |
92-98% |
90-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
