FineTest
SKU:EH14659
Human HIST3H3 (Histone H3.1t) ELISA Kit
Human HIST3H3 (Histone H3.1t) ELISA Kit
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Product Name
Human HIST3H3 (Histone H3.1t) ELISA Kit
Alias
Histone H3.1t ELISA Kit, H3/t ELISA Kit, H3t ELISA Kit, H3/g ELISA Kit, Histone H3.4 ELISA Kit, H3-4 ELISA Kit, H3FT ELISA Kit, HIST3H3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HIST3H3 ELISA Kit allows for the in vitro quantitative determination of HIST3H3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.1-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 °C for 6 months
Recovery
Matrices listed below were spiked with certain level of HIST3H3 and the recovery rates were calculated by comparing the measured value to the expected amount of HIST3H3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-104 |
96 |
|
EDTA plasma(n=5) |
89-104 |
98 |
|
heparin plasma(n=5) |
86-104 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIST3H3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-105% |
89-99% |
90-100% |
|
EDTA plasma(n=5) |
86-93% |
82-99% |
85-100% |
|
heparin plasma(n=5) |
80-98% |
81-92% |
87-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
