FineTest
SKU:EH10037
Human MED30 (Mediator complex subunit 30) ELISA Kit
Human MED30 (Mediator complex subunit 30) ELISA Kit
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Product Name
Human MED30(Mediator complex subunit 30) ELISA Kit
Alias
MED30 ELISA Kit, Mediator complex subunit 30 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MED30 ELISA Kit allows for the in vitro quantitative determination of MED30 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 °C for 6 months
Recovery
Matrices listed below were spiked with certain level of MED30 and the recovery rates were calculated by comparing the measured value to the expected amount of MED30 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-102 |
95 |
|
EDTA plasma(n=5) |
86-104 |
95 |
|
heparin plasma(n=5) |
87-99 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MED30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
89-105% |
86-101% |
|
EDTA plasma(n=5) |
84-99% |
82-99% |
85-96% |
|
heparin plasma(n=5) |
82-94% |
83-91% |
81-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
