FineTest
SKU:EH11808
Human RHEB (Ras homolog enriched in brain) ELISA Kit
Human RHEB (Ras homolog enriched in brain) ELISA Kit
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Product Name
Human RHEB(Ras homolog enriched in brain) ELISA Kit
Alias
GTP-binding protein Rheb ELISA Kit, Ras homolog enriched in brain ELISA Kit, RHEB ELISA Kit, RHEB2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
RHEB ELISA Kit allows for the in vitro quantitative determination of RHEB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of RHEB and the recovery rates were calculated by comparing the measured value to the expected amount of RHEB in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-99 |
95 |
|
EDTA plasma(n=5) |
98-103 |
100 |
|
heparin plasma(n=5) |
86-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of RHEB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-105% |
87-99% |
93-101% |
|
EDTA plasma(n=5) |
87-96% |
83-101% |
82-99% |
|
heparin plasma(n=5) |
81-90% |
82-95% |
82-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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