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Human MRPL53(Mitochondrial large ribosomal subunit protein mL53) ELISA Kit
Human MRPL53(Mitochondrial large ribosomal subunit protein mL53) ELISA Kit
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Product Name
Human MRPL53(Mitochondrial large ribosomal subunit protein mL53) ELISA Kit
Alias
39S ribosomal protein L53 ELISA Kit, mitochondrial ELISA Kit, L53mt ELISA Kit, MRP-L53 ELISA Kit, Mitochondrial large ribosomal subunit protein mL53 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MRPL53 ELISA Kit allows for the in vitro quantitative determination of MRPL53 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 °C for 6 months
Recovery
Matrices listed below were spiked with certain level of MRPL53 and the recovery rates were calculated by comparing the measured value to the expected amount of MRPL53 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
96 |
|
EDTA plasma(n=5) |
86-105 |
96 |
|
heparin plasma(n=5) |
86-101 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MRPL53 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-103% |
85-105% |
92-102% |
|
EDTA plasma(n=5) |
85-101% |
85-98% |
82-96% |
|
heparin plasma(n=5) |
84-100% |
84-100% |
81-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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