FineTest
SKU:EH15191
Human PYCARD(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit
Human PYCARD(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit
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Product Name
Human PYCARD(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit
Alias
hASC ELISA Kit, Caspase recruitment domain-containing protein 5 ELISA Kit, PYD and CARD domain-containing protein ELISA Kit, Target of methylation-induced silencing 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PYCARD ELISA Kit allows for the in vitro quantitative determination of PYCARD concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PYCARD and the recovery rates were calculated by comparing the measured value to the expected amount of PYCARD in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-105 |
99 |
|
EDTA plasma(n=5) |
89-101 |
95 |
|
heparin plasma(n=5) |
88-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PYCARD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
93-98% |
86-105% |
87-103% |
|
EDTA plasma(n=5) |
84-100% |
88-98% |
86-96% |
|
heparin plasma(n=5) |
85-87% |
81-98% |
80-91% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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