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SKU:EH15191

Human PYCARD(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit

Human PYCARD(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Human PYCARD(Apoptosis-associated speck-like protein containing a CARD) ELISA Kit

Alias

hASC ELISA KitCaspase recruitment domain-containing protein 5 ELISA KitPYD and CARD domain-containing protein ELISA KitTarget of methylation-induced silencing 1 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

PYCARD ELISA Kit allows for the in vitro quantitative determination of PYCARD concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000pg/ml

Sensitivity

< 46.875pg/ml

Species

Human

UniProt ID

Q9ULZ3

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PYCARD and the recovery rates were calculated by comparing the measured value to the expected amount of PYCARD in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

93-105

99

EDTA plasma(n=5)

89-101

95

heparin plasma(n=5)

88-104

97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PYCARD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

93-98%

86-105%

87-103%

EDTA plasma(n=5)

84-100%

88-98%

86-96%

heparin plasma(n=5)

85-87%

81-98%

80-91%

CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

 

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