FineTest
SKU:EHS0052
Horse IL-1β(Interleukin 1 Beta)ELISA Kit
Horse IL-1β(Interleukin 1 Beta)ELISA Kit
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Product Name
Horse IL-1β(Interleukin 1 Beta)ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-1βELISA Kit allows for the in vitro quantitative determination of IL-1β concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Horse
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-1β and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-103 |
99 |
|
EDTA plasma(n=5) |
87-99 |
92 |
|
heparin plasma(n=5) |
89-100 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-103% |
87-104% |
88-104% |
|
EDTA plasma(n=5) |
84-93% |
84-100% |
89-97% |
|
heparin plasma(n=5) |
85-99% |
80-100% |
84-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
