FineTest
SKU:EH14469
Human FTO(Alpha-ketoglutaRate-dependent dioxygenase FTO) ELISA Kit
Human FTO(Alpha-ketoglutaRate-dependent dioxygenase FTO) ELISA Kit
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Product Name
Human FTO(Alpha-ketoglutaRate-dependent dioxygenase FTO) ELISA Kit
Alias
FTO ELISA Kit, KIAA1752 ELISA Kit, fat mass and obesity associated ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
FTO ELISA Kit allows for the in vitro quantitative determination of FTO concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of FTO and the recovery rates were calculated by comparing the measured value to the expected amount of FTO in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-104 |
98 |
|
EDTA plasma(n=5) |
85-100 |
92 |
|
heparin plasma(n=5) |
86-105 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FTO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-98% |
85-103% |
93-105% |
|
EDTA plasma(n=5) |
85-95% |
85-100% |
90-101% |
|
heparin plasma(n=5) |
87-96% |
85-100% |
85-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
