FineTest
SKU:EH9991
Human MBD3(Methyl-CpG-binding domain protein 3) ELISA Kit
Human MBD3(Methyl-CpG-binding domain protein 3) ELISA Kit
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Product Name
Human MBD3(Methyl-CpG-binding domain protein 3) ELISA Kit
Alias
MBD3 ELISA Kit, Methyl-CpG-binding domain protein 3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MBD3 ELISA Kit allows for the in vitro quantitative determination of MBD3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of MBD3 and the recovery rates were calculated by comparing the measured value to the expected amount of MBD3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-98 |
93 |
|
EDTA plasma(n=5) |
90-105 |
99 |
|
heparin plasma(n=5) |
85-96 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MBD3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-98% |
95-101% |
85-103% |
|
EDTA plasma(n=5) |
82-101% |
85-99% |
86-97% |
|
heparin plasma(n=5) |
86-100% |
80-98% |
85-93% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
