FineTest
SKU:ER1728
Rat A1AT(alpha 1-Antitrypsin) ELISA Kit
Rat A1AT(alpha 1-Antitrypsin) ELISA Kit
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Product Name
Rat A1AT(alpha 1-Antitrypsin) ELISA Kit
Alias
α1-AT ELISA Kit, A1AT ELISA Kit, alpha 1-Antitrypsin ELISA Kit, Serpin A1 ELISA Kit, alpha 1-Proteinase Inhibitor ELISA Kit, A1A ELISA Kit, AATMGC23330 ELISA Kit, Alpha-1 protease inhibitor ELISA Kit, Alpha-1-antiproteinase ELISA Kit, alpha-1-antitrypsin ELISA Kit, antitrypsin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
A1AT ELISA Kit allows for the in vitro quantitative determination of A1AT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000ng/ml
Sensitivity
< 18.75ng/ml
Species
Rat
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of A1AT and the recovery rates were calculated by comparing the measured value to the expected amount of A1AT in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-103 |
94 |
|
EDTA plasma(n=5) |
85-102 |
94 |
|
heparin plasma(n=5) |
85-100 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of A1AT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-104% |
85-101% |
89-103% |
|
EDTA plasma(n=5) |
82-92% |
85-99% |
92-99% |
|
heparin plasma(n=5) |
84-98% |
86-100% |
82-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
