FineTest
SKU:EH11323
Human PPIF(Peptidyl-prolyl cis-trans isomerase F) ELISA Kit
Human PPIF(Peptidyl-prolyl cis-trans isomerase F) ELISA Kit
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Product Name
Human PPIF(Peptidyl-prolyl cis-trans isomerase F) ELISA Kit
Alias
Cyclophilin F ELISA Kit, Cyp D ELISA Kit, CYP3 ELISA Kit, peptidylprolyl isomerase F ELISA Kit, PPIase F ELISA Kit, PPIF ELISA Kit, Rotamase F ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PPIF ELISA Kit allows for the in vitro quantitative determination of PPIF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PPIF and the recovery rates were calculated by comparing the measured value to the expected amount of PPIF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-105 |
98 |
|
EDTA plasma(n=5) |
86-100 |
92 |
|
heparin plasma(n=5) |
92-103 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PPIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-104% |
92-105% |
86-98% |
|
EDTA plasma(n=5) |
87-96% |
89-101% |
84-100% |
|
heparin plasma(n=5) |
87-99% |
84-94% |
87-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
