FineTest
SKU:EB0170
Bovine IL-12A(Interleukin-12 subunit alpha) ELISA Kit
Bovine IL-12A(Interleukin-12 subunit alpha) ELISA Kit
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Product Name
Bovine IL-12A(Interleukin-12 subunit alpha) ELISA Kit
Alias
IL12A ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IL-12A ELISA Kit allows for the in vitro quantitative determination of IL12A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Bovine
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL12A and the recovery rates were calculated by comparing the measured value to the expected amount of IL12A in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-99 |
94 |
|
EDTA plasma(n=5) |
86-103 |
94 |
|
heparin plasma(n=5) |
90-96 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL12A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-105% |
96-102% |
85-103% |
|
EDTA plasma(n=5) |
82-97% |
89-101% |
83-101% |
|
heparin plasma(n=5) |
84-94% |
83-96% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
