FineTest
SKU:EH14188
Human CH25H(Cholesterol 25-hydroxylase) ELISA Kit
Human CH25H(Cholesterol 25-hydroxylase) ELISA Kit
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Product Name
Human CH25H(Cholesterol 25-hydroxylase) ELISA Kit
Alias
Cholesterol 25-hydroxylase ELISA Kit, H25oh ELISA Kit, CH25H ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
CH25H ELISA Kit allows for the in vitro quantitative determination of CEMIP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of CEMIP and the recovery rates were calculated by comparing the measured value to the expected amount of CEMIP in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-100 |
97 |
|
EDTA plasma(n=5) |
92-102 |
98 |
|
heparin plasma(n=5) |
87-103 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CEMIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
92-105% |
85-105% |
87-105% |
|
EDTA plasma(n=5) |
90-100% |
83-97% |
85-101% |
|
heparin plasma(n=5) |
82-95% |
82-94% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
