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SKU:EH9993
Human MBD5(Methyl-CpG-binding domain protein 5) ELISA Kit
Human MBD5(Methyl-CpG-binding domain protein 5) ELISA Kit
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Product Name
Human MBD5(Methyl-CpG-binding domain protein 5) ELISA Kit
Alias
KIAA1461 ELISA Kit, MBD5 ELISA Kit, MRD1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MBD5 ELISA Kit allows for the in vitro quantitative determination of MBD5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of MBD5 and the recovery rates were calculated by comparing the measured value to the expected amount of MBD5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-105 |
99 |
|
EDTA plasma(n=5) |
87-103 |
95 |
|
heparin plasma(n=5) |
86-103 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MBD5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
93-104% |
87-103% |
87-94% |
|
EDTA plasma(n=5) |
88-94% |
87-99% |
90-99% |
|
heparin plasma(n=5) |
81-100% |
84-99% |
84-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
