FineTest
SKU:ER1636
Rat Calprotectin(Calprotectin) ELISA Kit
Rat Calprotectin(Calprotectin) ELISA Kit
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Product Name
Rat calprotectin(S100A8/S100A9 Heterodimer)ELISA Kit
Alias
S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9 ELISA Kit, Calprotectin ELISA Kit, S100A8/S100A9 Heterodimer ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
calprotectinELISA Kit allows for the in vitro quantitative determination of S100A8/S100A9 Heterodimer concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
6.25-400ng/ml
Sensitivity
< 3.75ng/ml
Species
Rat
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of S100A8/S100A9 Heterodimer and the recovery rates were calculated by comparing the measured value to the expected amount of S100A8/S100A9 Heterodimer in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-95 |
91 |
|
EDTA plasma(n=5) |
85-100 |
92 |
|
heparin plasma(n=5) |
87-99 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100A8/S100A9 Heterodimer and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-105% |
85-104% |
88-98% |
|
EDTA plasma(n=5) |
86-98% |
82-92% |
85-94% |
|
heparin plasma(n=5) |
88-94% |
80-98% |
84-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
